1
عمومی::
آنتیبادی پلیکلونال
, Sandwich ELISA using an anti-human polyclonal antibody), and dividing the antibody concentration in the brain by the antibody concentration in the plasma.
[00339] Sandwich ELISA with anti-human IgG polyclonal antibodies was used to determine plasma antibody concentrations.
[00341] A 1 �g/mL PBS solution of goat anti-human IgG (Fc) polyclonal antibodies was pipetted into a 96-well plate (MaxSorp (NUNC)) and allowed to stand at 4�C overnight.
Then, blocking was accomplished with 3% BSA/PBS to prepare a plate with immobilized goat anti-human IgG (Fc) polyclonal antibodies.
The plasma and standard substance were diluted with 3% BSA/PBS and pipetted into the plate with immobilized goat anti-human IgG (Fc) polyclonal antibodies at 50 �L/well.
واژگان شبکه مترجمین ایران